Journal: Journal of Personalized Medicine

Article Title: Effects of the Autophagy-Inhibiting Agent Chloroquine on Acute Myeloid Leukemia Cells; Characterization of Patient Heterogeneity

doi: 10.3390/jpm11080779

Figure Lengend Snippet: The effect of chloroquine (CQ) on the proliferation of primary AML cells and normal MSCs grown in coculture. The two cell populations were separated by a semipermeable membrane. Cocultures of AML cells (14 patients tested) and normal MSCs derived from a healthy donor were treated with 5 µM chloroquine for three days. Proliferation was measured using the 3H-thymidine incorporation assay for both AML cells and MSCs in cocultures, with and without chloroquine-treatment. Each dot indicates the cell proliferation of AML or MSCs after coculture, where results are shown as percent proliferation of chloroquine-treated cocultures compared to untreated cocultures, with the median level. Chloroquine inhibited the proliferation of both AML cells and MSCs in cocultures.

Article Snippet: Leukemia cell preparation was performed using density gradient separation (density 1.077 g/mL; Lymphoprep, Serumwerk Bernburg AG for Alere Technologies AS, Oslo, Norway), resulting in cell populations with >90% leukemia cells for all patients, where contaminating cells were mainly small lymphocytes [ , , ].

Techniques: Membrane, Derivative Assay, Thymidine Incorporation Assay